Published February 21, 2023
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Phytochemical analysis, antioxidant and hypoglycemic activities of a methanol extract from Stachys brachyclada de Noé ex Coss. leaves.

Creators

  • 1. Département de Physique et Chemique, Ecole Normale Supérieure, Constantine, Algérie.
  • 2. École Normale Supérieure
  • 3. Unité de Recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques, Université Frères Mentouri, Constantine, Algérie.
  • 4. Department of Pharmacy, University of Salerno, Fisciano, Italy.
  • 5. Laboratoire de Synthèse Organique, Modélisation et Optimisation des Procèdes (LOMOP), Faculté des Sciences, Département de Chimie, Université Badji Mokhtar, Annaba, Algérie.
  • 6. Unesco Chair Salerno, Plantae Medicinales Mediterraneae, University of Salerno, Fisciano, Italy.
  • 7. Department of Science, University of Basilicata, Potenza, Italy.
  • 8. Faculty of Clinical Pharmacy, 21 September University of Medical and Applied Sciences, Sana'a, Yemen.
  • 9. Ecology and Environment Department, Khenchela University, Khenchela, Algeria.

Description

Stachys brachyclada de Noé ex Coss. (Lamiaceae) is a quite rare medicinal plant endemic to the Mediterranean basin. In this study, seven secondary metabolites from a methanol extract of its leaves have been isolated and identified by a combination of chromatographic and spectroscopic methods (1D and 2D NMR experiments and ESIMS analysis). They include one ethyl 4-hydroxybenzoate (1), three acylated flavone glycosides (2-4), one diapigenin derivative (5) and two flavone aglycones (6-7). Stachysetin (5) was found the major compound of the extract (74.0 mg/g of dry matter). Moreover, the produced extract showed the ability in inhibiting the α-glucosidase enzyme (IC50 = 13.7 µg/mL), in quenching the radical 1,1-diphenyl-2-picrylhydrazyl (EC50 = 74.6 µg/mL), and in reducing the intracellular oxidative stress level in Human Dermal Fibroblast (64% inhibition at 50 µg/mL).
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