Synthesis, Characterization, Cytotoxicity, and Antibacterial Studies of Persea americana Mill. (Avocado) Seed Husk Mediated Hydronium Jarosite Nanoparticles

The application of nanotechnology in antimicrobial and cytotoxicity studies has recently been receiving increased interest. This paper report on the use of Persea americana Mill. (avocado) seed husk to synthesize hydronium jarosite nanoparticles in a facile, economical, and eco-friendly manner. We describe firstly the synthesis of hydronium jarosite nanoparticles using P. americana (avocado) seed husk aqueous extract as a reducing and chelating agent for the reduction of iron (II) sulfate heptahydrate. Secondly, we describe the characterization of the nanoproduct with scanning electron microscopy (SEM); energy dispersive X-ray spectroscopy (EDX); high-resolution transmission electron microscopy (HRTEM); X-ray powder diffraction (XRD) analysis; Fourier transform infrared spectroscopy (FT-IR); and, lastly, the cytotoxicity and antibacterial effect of hydronium jarosite nanoparticles using murine macrophage cells (Raw 264.7) cell lines, Gram-negative (Escherichia coli), Gram-positive (Staphylococcus aureus), and methicillin-resistant Staphylococcus aureus. These hydronium jarosite avocado seed husk-mediated nanoparticle-coated 2D sheets did not show any antibacterial activity against the bacteria tested but did show concentration-dependent cytotoxicity. Further research is required to optimize the antibacterial properties and reduce the cytotoxicity of this nanomaterial synthesized using green nanochemistry.