Generation of human pluripotent stem cell lines with suppressed expression of the Notch ligand DLL4 using short hairpin RNAs.
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- 1. University of Granada
- 2. Pfizer
Description
Abstract Studies with different animal models have shown that the Notch ligand DLL4 has a key role in the development of the embryonic vasculature. Here we describe the generation and characterization of a human embryonic stem cell line and an induced pluripotent stem cell line that constitutively express short hairpin RNAs targeting DLL4 mRNA. These cells present reduced DLL4 expression at both protein and mRNA level, as well as a reduced induction of DLL4 target genes. They represent an ideal tool to study the role of DLL4 in human embryonic vascular and hematopoietic development.
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Financial Support
Fundacion Publica Andaluza Progreso y Salud — Grant: PI-0030-2014
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Ministry of Economy and Competitiveness — Grant: JCI_2012_12666
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Marie Curie Intra-European Fellow — Grant: FP7-MC-IEF-623806
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Fundacion Inocente Inocente — Grant: FII2015-212
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FIS/FEDER — Grant: CP09/0063
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FIS/FEDER — Grant: PI12/01596
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FIS/FEDER — Grant: CPII15/00018
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FIS/FEDER — Grant: CP12/03175
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FIS/FEDER — Grant: PI14/01412
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L'Oréal-UNESCO
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References
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Duarte . Dosage-sensitive requirement for mouse Dll4 in artery development, Gene...
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Ramos-Mej\u00eda . HOXA9 promotes hematopoietic commitment of human embryonic st...
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Benedito . Loss of Notch signalling induced by Dll4 causes arterial calibre redu...
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Krebs . Haploinsufficient lethality and formation of arteriovenous malformations...
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