Life cycle assessment and yield to optimize extraction time and solvent: Comparing deep eutectic solvents vs conventional ones.

Deep eutectic solvents (DES) are gaining interest as eco-friendly alternatives for extracting bioactive compounds, but their environmental benefits remain unclear and need further evaluation. In this work, a case study of total polyphenols (TPC) extraction from spent coffee grounds (SCG) was environmentally evaluated using life cycle assessment (LCA). First, the most convenient extraction time (1, 10, 20, or 40 min) for water and acetone 20 % from an environmental perspective was identified. Second, a comparison of different solvents-DES (choline chloride-1,6-hexanediol), water, and ethanol 20 %-under their optimal extraction yield conditions was performed using literature data. Results from the first study revealed that the environmentally optimal extraction time (10 min) was not the one leading to the highest yield. The main contributors to the impacts were the use of acetone and electricity consumption. For the second study, DES performed worse in all studied environmental impact categories compared to both ethanol 20 % and water. Ethanol 20 % was the better option compared to water due to its higher extraction yield (9.2 mg vs. 6.5 mg TPC/g SCG, respectively). The environmental impacts associated with the DES system were primarily attributed to the DES preparation step, which requires virgin raw materials (e.g., dimethyl hexanediol), and the adsorption stage involving the use of resins. A sensitivity analysis was also conducted by optimizing the DES system to the best possible described conditions (90 % reuse of DES and maximum reduction of the macroporous resin used to adsorb the TPC after extraction). Nevertheless, the DES system still performed worse than water or ethanol 20 % systems, in 11 out of 16 impact categories. The study highlights the importance to consider environmental impacts and yield when optimizing extraction processes, especially at the laboratory scale, as the insights gained are valuable for improving eco-efficiency on an industrial scale.