Published January 21, 2021
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Physiochemical properties and antibacterial activity of silver nanoparticles green synthesized by Camellia sinensis and Prunus africana extracts

Creators

  • 1. College of Veterinary Medicine Animal Resources and Biosecurity, Makerere University P. O. Box 7062 Kampala Uganda
  • 2. UNESCO-UNISA Africa Chair in Nanosciences/Nanotechnology, College of Graduate Studies, University of South Africa (UNISA), Muckleneuk Ridge, PO Box 392, Pretoria, South Africa
  • 3. Nanosciences African Network (NANOAFNET), iThemba LABS-National Research Foundation, Old Faure Road, 7129 Somerset West, South Africa
  • 4. University of South Africa-Florida Campus Private Bag X6 Florida 1710
  • 5. African Center of Excellence in Materials, Product Development and Nanotechnology, College of Engineering, Design, Art and Technology, Makerere University P. O. Box 7062, Kampala Uganda

Description

Abstract Antibiotics have been the nucleus of chemotherapy since their discovery and introduction into the healthcare system in the 1940s. They are used routinely not only to treat bacterial infections but also to prevent infections in patients with compromised immune systems and enhancing growth in livestock. However, resistance to last-resort antibiotics used in the treatment of MDR infections has been reported worldwide. Therefore, the aim of this study was to evaluate green synthesized nanomaterials such as AgNPs as alternatives to antibiotics. UV Vis Spectroscopy surface plasmon resonance peaks for AgNPs were obtained between 417 to 475nm. XRD analysis generated 4 peaks for both PAE and CSE biosynthesized AgNPs positioned at 2θ angles of 38.2˚, 44.4˚, 64.5˚, and 77.4˚ corresponding to crystal planes (111), (200), (220) and (311) respectively. DLS registered mean zeta potential of + 6.3mV and + 0.9mV for PAE and CSE biosynthesized nanoparticles respectively. FTIR spectra exhibited bands corresponding to different organic functional groups confirming capping of AgNPs by PAE and CSE phytochemicals. FESEM imaging showed that AgNPs were spherical with average size distribution ranging from 10 to 19nm. Biosynthesized AgNPs exhibited maximum growth inhibitory zones of 21mm with MIC and MBC of 125µg/ml and 250µg/ml respectively against carbapenem resistant bacteria.
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